LF-880 spectrometer with 2.0nm resolution
Measuring Chlorophyll Fluorescence as an
Indication of Plant Health
 First in Full Range PDA Spectrometer, Spectroradiometer and Spectrophotometer Systems
1 Canal Street  ◊  Unit B1
Lawrence, MA 01840  USA
Tel: 978 687-1833  ◊  Fax: 978 945-0372

Copyright © 2012 Spectral Evolution, Inc
NIR spectroscopy can be used to measure chlorophyll fluorescence
for determining the nutritional status of plants, as well as the
presence of nutrients in water bodies. SPECTRAL EVOLUTION'S          
SM-3500 full range UV/VIS/NIR field portable agricultural
spectrometer and the LF-880 lab spectrometer can both be used to
perform chlorophyll measurements.

A green laser in the 530nm range can be used to excite the
a and b molecules to fluoresce. The spectrometer can
capture the scan showing the peaks. As samples are scanned, the
difference between healthy samples and distressed samples can be
seen in the difference in amplitude between the a and b chlorophyll

Measuring chlorophyll fluorescence can indicate pl;ants that are
stressed by lack of water, disease, or insect infestation. These
measurements can be performed on forest canopies, crops, or single
leaf samples.

For water samples, chlorophyll fluorescence can be used to
determine water quality. Chlorophyll is essential to the existence of
phytoplankton and algae. Monitoring chlorophyll levels can help
researchers track algal growth, including blooms, and its influence
on algal growth. Too much growth or bloom and subsequent crash
depletes the dissolved oxygen level and leads to most fish kills.
These blooms are most often the result of high chlorophyll
conditions and high nutrients that can be caused by leaking septic
systems, poorly functioning wastewater treatment plants, or fertilizer

Measuring chlorophyll fluorescence using NIR spectroscopy is faster
and more cost effective that using grab samples sent to a lab.

The following images show examples of spectra collected by our
spectrometers from healthy and distressed foliage.
 First in Full Range PDA Spectrometer, Spectroradiometer and Spectrophotometer Systems
LF-880 NIR spectrometer for chlorophyll fluorescence measurement
The LF-880 is a compact,
lightweight spectrometer
ideal for lab use. It
provides auto-shutter and
auto-exposure capabilities
for one touch operation.
With no moving optics it
delivers accurate
measurements with high
SM-3500 is a full range spectrometer for vegetation research
The SM-3500 is a field
portable, full range with a
spectral range of
350-2500nm, ideal for
vegetation, forest canopy,
water body, climate, and
agricultural research.
SM-3500 spectrometer with 3.5nm resolution