Measuring Cellulose Content in Vegetation

Using a field spectroradiometer such as the Spectral Evolution SR-6500, NaturaSpec™, PSR+ or the RS-3500, a researcher can determine cellulose content in plants or biomass for critical information on plant health or biomass characteristics. An NIR field spectroradiometer like the SR-6500, RS-5400, PSR+ or RS- 3500 can measure cellulose fast, with no sample prep, non- destructively in the field.

Determination of cellulose content is traditionally done with time- consuming and costly wet chemistry. With an SR-6500, NaturaSpec™, PSR+ or RS-3500, the process begins with scans of the plant or biomass material. NIR analysis for cellulose relies on absorption features created by chemical bonds that include C-O, O-H, C-H, and N-H . Common absorption peaks indicating cellulose in an SR-6500, NaturaSpec™, PSR+ or RS-3500 spectra from 350-2500nm can include the following:

  • A broad absorption peak at around 1216nm from C-H stretching
  • Several possible features around 1490nm—O-H stretching
  • Features between 1620 and 1780 nm attributable to O-H stretching
  • Feature around 1920-1930nm from O-H and C-O groups
  • Feature at 2100-2110nm is from O-H and C-H stretching
  • Feature at 2270-2272nm is from O-H and C-O
  • Feature at 2336 belongs to C-H stretching

Every Spectral Evolution instrument includes DARWin SP Data Acquisition software with 19 built-in vegetation indices, including PRI and the Red/Green index.