Academic Study- Assessment Physiological State
In this study, the evidence of a relation ship between the physiological state and leaf optical properties led us to investigate the behaviour of two species – Betula pendula Roth and Carpinus betulus L. – in order to determine if the vegetation is affected by the heavy metal load of the soil from a former mining area, Rosia Montana, Romania (46◦ 18 47 N, 23◦ 10 15 E). Two non-destructive techniques (leaf transmittance and reflectance) were compared to assess the physiological state of the plants, and 27 vegetation in dices for estimating foliar pigment content were tested. For chlorophyll assessment, the reflectance ratios RNIR/R550 and RNIR/R700 were the most reliable for an accurate estimation of the pigment content of the leaves. The light use efficiency (LUE) indices were within normal values, except the Red Green Ratio, indicating that the overall reactions of photosynthesis are not impaired by the specific conditions of the habitat. Differences in leaf optical properties are minimal between the two studied species and the values of the vegetation indices are showing similar trends. Both techniques performed consistently well and yielded accurate estimations of pigment content, supporting their interoperability.
Authors: Cristina Dobrota, Laura Lazar, Calin Baciu
Keywords: Light use efficiency, Heavy metals, Reflectance spectroscopy, Plant stress, Vegetation indices
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